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Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes
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JoVE Journal Genetik
Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

7,578 Views

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08:46 min

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March 03, 2020

DOI:

10.3791/60671-v

DOI:
10.3791/60671-v

•

08:46 min
March 03, 2020

•

8 Views
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1Department of Molecular and Cellular Biology,University of California, Davis, 2Integrative Genetics and Genomics Graduate Group,University of California, Davis

Özet

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Nuclear surface spreads are an indispensable tool for studying chromosome events during meiosis. Here we demonstrate a method to prepare and visualize meiotic chromosomes during prophase I from zebrafish spermatocytes.

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Meiotic Chromosome SpreadsZebrafish SpermatocytesVertebrate ModelMeiosisHomologous Chromosome PairingSynapsisRecombinationSuper Resolution MicroscopyDissectionTestesCollagenaseDMEMSucrose SolutionCell Dissociation

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Cite this Article

Blokhina, Y. P., Olaya, I., Burgess, S. M. Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes. J. Vis. Exp. (157), e60671, doi:10.3791/60671 (2020).

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