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RING-E3型无二体气体在Vitro和Planta中的功能特性
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
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RING-E3型无二体气体在Vitro和Planta中的功能特性

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10:27 min

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December 05, 2019

DOI:

10.3791/60533-v

DOI:
10.3791/60533-v

•

10:27 min
December 05, 2019

•

5 Views
, , , , , ,
1Department of Entomology, Plant Pathology and Nematology,University of Idaho, 2Department of Plant Sciences,University of Idaho

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本手稿的目的是为 RING 型 E3 泛性白化酶的综合生化和功能研究提供大纲。此多步骤管道，具有详细的协议，验证被测试蛋白质的酶活性，并演示如何将活动与功能联系起来。

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Cite this Article

Kud, J., Wang, W., Yuan, Y., Caplan, A., Kuhl, J. C., Dandurand, L., Xiao, F. Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta. J. Vis. Exp. (154), e60533, doi:10.3791/60533 (2019).

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