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Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

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10:27 min

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December 05, 2019

DOI:

10.3791/60533-v

DOI:
10.3791/60533-v

•

10:27 min
December 05, 2019

•

5 Views
, , , , , ,
1Department of Entomology, Plant Pathology and Nematology,University of Idaho, 2Department of Plant Sciences,University of Idaho

Summary

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The goal of this manuscript is to present an outline for the comprehensive biochemical and functional studies of the RING-type E3 ubiquitin ligases. This multistep pipeline, with detailed protocols, validates an enzymatic activity of the tested protein and demonstrates how to link the activity to function.

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Kud, J., Wang, W., Yuan, Y., Caplan, A., Kuhl, J. C., Dandurand, L., Xiao, F. Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta. J. Vis. Exp. (154), e60533, doi:10.3791/60533 (2019).

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