/
/
A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development
6,534 Views
•
06:49 min
•
October 29, 2019
DOI:
1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine,University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program,University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research,National Institutes of Health, 6Biyoloji Bölümü,San Diego State University, 7Developmental and Stem Cell Biology Graduate Program,University of California, San Francisco
Özet
Automatically generated
This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.
Read Article
Cite this Article
Khaliullin, R. N., Hendel, J. M., Gerson-Gurwitz, A., Wang, S., Ochoa, S. D., Zhao, Z., Desai, A., Oegema, K., Green, R. A. A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development. J. Vis. Exp. (152), e60362, doi:10.3791/60362 (2019).
Copy