Dergi
/
Nörobilim
/
Cochlear Surface Preparation in the Adult Mouse
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Cochlear Surface Preparation in the Adult Mouse

Cochlear Surface Preparation in the Adult Mouse

14,927 Views

•

09:51 min

•

November 06, 2019

DOI:

10.3791/60299-v

DOI:
10.3791/60299-v

•

09:51 min
November 06, 2019

•

36 Views
, , ,
1Department of Pathology and Laboratory Medicine,Medical University of South Carolina, 2MOE Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences,Southeast University

Özet

Automatically generated

This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae.

Explore More Videos

Cochlear Surface PreparationImmunohistochemistryConfocal ImagingCochlear MicrodissectionCochlear EpitheliumHair Cell RegenerationTemporal Bone ExtractionCochlear FixationEDTA Decalcification

Read Article

Cite this Article

Fang, Q., Wu, F., Chai, R., Sha, S. Cochlear Surface Preparation in the Adult Mouse. J. Vis. Exp. (153), e60299, doi:10.3791/60299 (2019).

Download .ris file

Copy

Share Video

.

Copy