The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.
Temkin, M. I., Carlson, C. M., Stubbendieck, A. L., Currie, C. R., Stubbendieck, R. M. High Throughput Co-culture Assays for the Investigation of Microbial Interactions. J. Vis. Exp. (152), e60275, doi:10.3791/60275 (2019).