Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.
Davis, M. J., Talbot, D., Jemc, J. Assay for Blood-brain Barrier Integrity in Drosophila melanogaster. J. Vis. Exp. (151), e60233, doi:10.3791/60233 (2019).