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SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

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08:29 min

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November 01, 2019

DOI:

10.3791/60098-v

DOI:
10.3791/60098-v

•

08:29 min
November 01, 2019

•

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1Liver Disease and Liver Metabolism Lab,CIC bioGUNE, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), 2Proteomics Platforms,CIC bioGUNE, ProteoRED-ISCIII, CIBERehd, 3Advanced Technology Institute in Life Sciences (ITAV)-CNRS-IPBS,UbiCARE

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Here, we present a protocol to enrich, isolate, identify, and characterize proteins modified by SUMO in vivo both from human hepatoma cells and liver tumors obtained from mouse models of hepatocellular carcinoma by using SUMO-binding entities (SUBEs).

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Lopitz-Otsoa, F., Delgado, T. C., Lachiondo-Ortega, S., Azkargorta, M., Elortza, F., Rodríguez, M. S., Martínez-Chantar, M. L. SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer. J. Vis. Exp. (153), e60098, doi:10.3791/60098 (2019).

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