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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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09:33 min
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October 03, 2019
DOI:
1Centre for Craniofacial and Regenerative Biology,King’s College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research,University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics,King’s College London, 4Department of Biological Applications and Technology,University of Ioannina
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This protocol describes the dissection and culture of cranial neural crest cells from mouse models, primarily for the study of cell migration. We describe the live imaging techniques used and the analysis of speed and cell shape changes.
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Gonzalez Malagon, S. G., Dobson, L., Muñoz, A. M. L., Dawson, M., Barrell, W., Marangos, P., Krause, M., Liu, K. J. Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration. J. Vis. Exp. (152), e60051, doi:10.3791/60051 (2019).
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