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在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
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在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试

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11:31 min

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August 16, 2019

DOI:

10.3791/59985-v

DOI:
10.3791/59985-v

•

11:31 min
August 16, 2019

•

2 Views
, , , , , ,
1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Medicine, Endocrinology, Diabetes & Metabolism,Baylor Tıp Fakültesi, 3Scientific Center,Shandong Provincial Hospital affiliated to Shandong University

Özet

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本文介绍了一种用于生成抗原特异性CD8 T细胞及其在体外扩张的协议，目的是产生大量功能性T细胞，用于体外和体内。

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Davidson, H. W., Cepeda, J. R., Sekhar, N. S., Han, J., Gao, L., Sosinowski, T., Zhang, L. High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model. J. Vis. Exp. (150), e59985, doi:10.3791/59985 (2019).

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