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高密度DNA和RNA微阵列.光刻合成、杂交和制备大型核酸库
JoVE Journal
Genetik
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JoVE Journal Genetik
High-Density DNA and RNA microarrays – Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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高密度DNA和RNA微阵列.光刻合成、杂交和制备大型核酸库

17,085 Views

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11:22 min

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August 12, 2019

DOI:

10.3791/59936-v

DOI:
10.3791/59936-v

•

11:22 min
August 12, 2019

•

15 Views
, , , ,
1Institute of Inorganic Chemistry, Faculty of Chemistry,University of Vienna

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Automatically generated

本文提出并讨论了原位制造核酸微阵列的合成和应用的新进展。具体来说,我们展示了DNA合成的规程如何扩展到RNA,以及如何利用微阵列创建可检索的核酸库。

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Lietard, J., Schaudy, E., Hölz, K., Ameur, D., Somoza, M. M. High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries. J. Vis. Exp. (150), e59936, doi:10.3791/59936 (2019).

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