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Identificatie van voetafdrukken van RNA: eiwitcomplexen via RNA-immunoprecipitatie in tandem gevolgd door sequencing (RIPiT-SEQ)
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JoVE Journal Genetik
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
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Identificatie van voetafdrukken van RNA: eiwitcomplexen via RNA-immunoprecipitatie in tandem gevolgd door sequencing (RIPiT-SEQ)

10,091 Views

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09:26 min

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July 10, 2019

DOI:

10.3791/59913-v

DOI:
10.3791/59913-v

•

09:26 min
July 10, 2019

•

11 Views
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1Department of Molecular Genetics, Center for RNA Biology,The Ohio State University

Özet

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Hier presenteren we een protocol om endogene RNA binding sites of "Footprints" van RNA: proteïne (RNP) complexen uit zoogdiercellen te verrijken. Deze aanpak omvat twee immunoprecipitaties van RNP-subeenheden en wordt daarom nagesynchroniseerde RNA-immunoprecipitatie in tandem (RIPiT).

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Woodward, L., Gangras, P., Singh, G. Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq). J. Vis. Exp. (149), e59913, doi:10.3791/59913 (2019).

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