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Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
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Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine
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June 24, 2019
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We describe a method to visualize GFP-labeled γδ IELs using intravital imaging of murine small intestine by inverted spinning disk confocal microscopy. This technique enables the tracking of live cells within the mucosa for up to 4 h and can be used to investigate a variety of intestinal immune-epithelial interactions.
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Cite this Article
Jia, L., Edelblum, K. L. Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine. J. Vis. Exp. (148), e59853, doi:10.3791/59853 (2019).
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