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rRNA 2'-O-甲基化的脱氧核糖核酸依赖性分析
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
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rRNA 2'-O-甲基化的脱氧核糖核酸依赖性分析

8,009 Views

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09:12 min

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September 16, 2019

DOI:

10.3791/59700-v

DOI:
10.3791/59700-v

•

09:12 min
September 16, 2019

•

31 Views
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1School of Biosciences,University of Birmingham

Özet

Automatically generated

在这里，我们提出了RNADNA依赖性裂解的协议。这使得RNA2'-O-甲基化能够进行快速和位点依赖性分析。这种方法可用于对打用RNA活性的初步或主要评估。

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Cite this Article

Winczura, K., Grzechnik, P. DNAzyme-dependent Analysis of rRNA 2’-O-Methylation. J. Vis. Exp. (151), e59700, doi:10.3791/59700 (2019).

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