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DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
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September 16, 2019
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Here we present a protocol for DNAzyme-dependent cleavage of RNA. This enables fast and site-dependent analysis of RNA 2’-O-methylation. This approach can be used for the preliminary or major assessment of snoRNA activity.
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Winczura, K., Grzechnik, P. DNAzyme-dependent Analysis of rRNA 2’-O-Methylation. J. Vis. Exp. (151), e59700, doi:10.3791/59700 (2019).
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