This method describes the culture of iPSC-derived endothelial cells as 40 perfused 3D microvessels in a standardized microfluidic platform. This platform enables the study of gradient-driven angiogenic sprouting in 3D, including anastomosis and stabilization of the angiogenic sprouts in a scalable and high-throughput manner.
van Duinen, V., Stam, W., Borgdorff, V., Reijerkerk, A., Orlova, V., Vulto, P., Hankemeier, T., van Zonneveld, A. J. Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro. J. Vis. Exp. (153), e59678, doi:10.3791/59678 (2019).