/
/
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
7,011 Views
•
09:40 min
•
June 07, 2019
DOI:
1Department of Biological Sciences,University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute,Children’s Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology,University of Southern California, Los Angeles
Özet
Automatically generated
We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.
Read Article
Cite this Article
Tran, M., Dave, M., Lansford, R. Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation. J. Vis. Exp. (148), e59664, doi:10.3791/59664 (2019).
Copy