Dergi
/
İmmünoloji ve Enfeksiyon
/
Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

DOI:
10.3791/59632-v

•

10:31 min

•

August 15, 2019

•

, , , , ,
1Department of Molecular Microbiology and Immunology,Saint Louis University, 2School of Veterinary Medicine University of California Davis

Bölümler

  • 00:04Başlık
  • 01:28Organ Isolation
  • 03:06Zika Virus Focus Forming Assay (FFA)
  • 08:05Results: Viral Replication and Focus Forming Assay
  • 09:39Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The goal of the protocol is to demonstrate the techniques used to investigate viral disease by isolating and quantifying Zika virus, from multiple organs in a mouse following infection.

Etiketler

Zika VirusViral InfectionMouse ModelCentral Nervous SystemOrgan HarvestingViral PathogenesisViral SpreadImmunological ResponseFlavivirusAlphavirusDengue VirusChikungunya VirusViral Quantification

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Isolation of Lymphocytes from Mouse Genital Tract Mucosa

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Zika Virus Specific Diagnostic Epitope Discovery

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Read Article