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Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.' 'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
'Bölümler'
- 00:04'Başlık'
- 00:58Alignment of the Microscope Spatial Beam Overlap
- 01:58Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
- 03:24Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
- 04:44Optimization of Temporal Synchronization of Beams
- 05:48Optimization of Spatial Overlap of Beams – Transmission Image Acquisition of OPO and Ti:Sa
- 06:48SRS Image Acquisition
- 07:40Results: Nonlinear Microscope Based on Stimulated Raman Scattering
- 08:43Conclusion
In this manuscript, the implementation of a stimulated Raman scattering (SRS) microscope, obtained by the integration of an SRS experimental set-up with a laser scanning microscope, is described. The SRS microscope is based on two femtosecond (fs) laser sources, a Ti-Sapphire (Ti:Sa) and synchronized optical parametric oscillator (OPO).
