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Determining Bile Duct Density in the Mouse Liver
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JoVE Journal Gelişim Biyolojisi
Determining Bile Duct Density in the Mouse Liver

Determining Bile Duct Density in the Mouse Liver

DOI:
10.3791/59587-v

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07:35 min

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April 30, 2019

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,
1Program in Developmental Biology,Baylor Tıp Fakültesi, 2Medical Scientist Training Program (MSTP),Baylor Tıp Fakültesi, 3Department of Molecular and Human Genetics,Baylor Tıp Fakültesi

Bölümler

  • 00:04Başlık
  • 00:36Mouse Liver Tissue Collection
  • 01:19Fixation, Paraffin Embedding, and Liver Block Sectioning
  • 02:35Immunohistochemistry for Wide-spectrum Cytokeratin (wsCK) and αSMA
  • 04:08Imaging and Quantification of Bile Ducts (BDs)
  • 05:27Results: Determining BD to PV Ratio
  • 07:00Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present a rather simple and sensitive method for accurate quantification of bile duct density in the mouse liver. This method can aid in determining the effects of genetic and environmental modifiers and the effectiveness of potential therapies in mouse models of biliary diseases.

Etiketler

Bile Duct DensityMouse LiverLiver DiseaseGenetic ModifiersBile Duct DevelopmentImmunohistochemistryParaffin EmbeddingLiver SectioningAntigen Retrieval

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