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Genotyping of Sea Anemone during Early Development
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Genotyping of Sea Anemone during Early Development

Genotyping of Sea Anemone during Early Development

DOI:
10.3791/59541-v

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07:04 min

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May 13, 2019

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,
1Department of Biological Sciences,University of Arkansas

Bölümler

  • 00:04Başlık
  • 00:36Induction of Spawning, In Vitro Fertilization, and De-jellying
  • 01:48Surgical Removal of an Aboral Tissue from a Gastrula Embryo
  • 03:38Genomic DNA Extraction and Genotyping PCR
  • 04:54Results: Genotyping Results of Sea Anemone Embryos
  • 06:07Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The goal of this protocol is to genotype the sea anemone Nematostella vectensis during gastrulation without sacrificing the embryo.

Etiketler

GenotypingSea AnemoneEarly DevelopmentMutantEmbriyogenezPhenotypeNematostella VectensisSpawningFertilizationDejellyDNA ExtractionAgarose GelMicrosurgery

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