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原子吸纳光谱测量哺乳动物细胞细胞内锌池
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JoVE Journal Biyokimya
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
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原子吸纳光谱测量哺乳动物细胞细胞内锌池

38,198 Views

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13:04 min

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May 16, 2019

DOI:

10.3791/59519-v

DOI:
10.3791/59519-v

•

13:04 min
May 16, 2019

•

30 Views
, , , , ,
1Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Kimya Bölümü,Skidmore College, 3Candiac MR Center,Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Özet

Automatically generated

培养的原生细胞系或已建立的细胞系通常用于在使用动物模型之前解决基本的生物和机械问题, 作为初步方法。该方案介绍了如何利用原子吸收光谱制备用于锌 (Zn) 和其他微量元素研究的全细胞提取物和亚细胞组分。

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Cite this Article

Gordon, S. J., Xiao, Y., Paskavitz, A. L., Navarro-Tito, N., Navea, J. G., Padilla-Benavides, T. Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells. J. Vis. Exp. (147), e59519, doi:10.3791/59519 (2019).

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