Journal
/
生物化学
/
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

38,209 Views

•

13:04 min

•

May 16, 2019

DOI:

10.3791/59519-v

DOI:
10.3791/59519-v

•

13:04 min
May 16, 2019

•

30 Views
, , , , ,
1Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Department of Chemistry,Skidmore College, 3Candiac MR Center,Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Summary

Automatically generated

Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.

Explore More Videos

Atomic Absorbance SpectroscopyIntracellular ZincMammalian CellsTransition MetalsMetal HomeostasisGraphite Furnace Atomic Absorption Spectroscopy (GF-AAS)Trace Element AnalysisMetal MisbalancesPathological ConditionsMethod DevelopmentSample PreparationLow Sample VolumeMethod Validation

Read Article

Cite this Article

Gordon, S. J., Xiao, Y., Paskavitz, A. L., Navarro-Tito, N., Navea, J. G., Padilla-Benavides, T. Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells. J. Vis. Exp. (147), e59519, doi:10.3791/59519 (2019).

Download .ris file

Copy

Share Video

.

Copy