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A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
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Genetik
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JoVE Journal Genetik
A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

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08:27 min

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May 22, 2019

DOI:

10.3791/59449-v

DOI:
10.3791/59449-v

•

08:27 min
May 22, 2019

•

1 Views
, , ,
1Department of Biochemistry and Molecular Genetics,University of Virginia School of Medicine, 2Nörobilim Bölümü,University of Virginia School of Medicine

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Here, we describe a protocol to generate a viable female murine model with non-random X chromosome inactivation, i.e., the maternally-inherited X chromosome is inactive in 100% of the cells. We also describe a protocol to test feasibility, tolerability, and safety of pharmacological reactivation of the inactive X chromosome in vivo.

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Przanowski, P., Zheng, Z., Wasko, U., Bhatnagar, S. A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome. J. Vis. Exp. (147), e59449, doi:10.3791/59449 (2019).

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