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A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
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A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

12,334 Views

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12:49 min

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September 28, 2019

DOI:

10.3791/59444-v

DOI:
10.3791/59444-v

•

12:49 min
September 28, 2019

•

7 Views
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1Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology, 2Visual Computing Center,King Abdullah University of Science and Technology

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The described pipeline is designed for the segmentation of electron microscopy datasets larger than gigabytes, to extract whole-cell morphologies. Once the cells are reconstructed in 3D, customized software designed around individual needs can be used to perform a qualitative and quantitative analysis directly in 3D, also using virtual reality to overcome view occlusion.

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Calì, C., Kare, K., Agus, M., Veloz Castillo, M. F., Boges, D., Hadwiger, M., Magistretti, P. A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells. J. Vis. Exp. (151), e59444, doi:10.3791/59444 (2019).

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