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Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
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March 22, 2019
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1Japan Science and Technology Agency,PRESTO, 2Laboratory for Developmental Neurobiology,RIKEN Center for Brain Science, 3École Normale Supèrieure, Institut de Biologie de l’ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University
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Here we present a protocol for Ca2+ imaging in neurons and glial cells, which enables the dissection of Ca2+ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca2+ indicators.
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Bannai, H., Hirose, M., Niwa, F., Mikoshiba, K. Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators. J. Vis. Exp. (145), e59246, doi:10.3791/59246 (2019).
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