Dergi
/
Biyokimya
/
哺乳动物细胞周期内蛋白质激酶 rna 激活的 rna 相互作用研究
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Please note that all translations are automatically generated. Click here for the English version.

哺乳动物细胞周期内蛋白质激酶 rna 激活的 rna 相互作用研究

6,299 Views

•

10:05 min

•

March 05, 2019

DOI:

10.3791/59215-v

DOI:
10.3791/59215-v

•

10:05 min
March 05, 2019

•

5 Views
, ,
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST),Korea Advanced Institute of Science and Technology (KAIST)

Özet

Automatically generated

我们提出了利用 hela 细胞研究哺乳动物细胞周期中双链 rna 结合蛋白激酶 rna 激活 (pkr) 的 rna 相互作用的实验方法。该方法利用甲醛对交联 rna-pkr 复合物和免疫沉淀进行富集 pkr 约束 rna。这些 rna 可以通过高通量测序或 qrt-pcr 进一步分析。

Explore More Videos

RNA InteractorsProtein Kinase RNA-ActivatedMammalian Cell CycleCrosslinkingFormaldehydeImmunoprecipitationNeurodegenerative DiseaseParkinson’s DiseaseAlzheimer’s DiseaseDouble-stranded RNARNA-binding ProteinsPost-transcriptional RegulationGene ExpressionCell Cycle ArrestHeLa CellsThymidineNocodazole

Read Article

Cite this Article

Kim, S., Kang, M., Kim, Y. Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle. J. Vis. Exp. (145), e59215, doi:10.3791/59215 (2019).

Download .ris file

Copy

Share Video

.

Copy