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Un dispositivo de posicionamiento para la colocación de ratones durante la entrega de siRNA intranasal al sistema nervioso central
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System
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Un dispositivo de posicionamiento para la colocación de ratones durante la entrega de siRNA intranasal al sistema nervioso central

21,936 Views

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05:28 min

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August 15, 2019

DOI:

10.3791/59201-v

DOI:
10.3791/59201-v

•

05:28 min
August 15, 2019

•

43 Views
, , , ,
1Department of Bioengineering and Institute of Nanoscience and Technology,Hanyang University, 2Department of Internal Medicine, Section of Infectious Diseases,Yale University School of Medicine

Özet

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Aquí, presentamos un protocolo utilizando un dispositivo de posicionamiento del ratón que permite la colocación adecuada de ratones para la administración intranasal de una formulación de péptido-siRNA dirigida al cerebro que permite un silenciamiento eficaz del gen en el sistema nervioso central.

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Ullah, I., Chung, K., Beloor, J., Lee, S., Kumar, P. A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System. J. Vis. Exp. (150), e59201, doi:10.3791/59201 (2019).

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