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Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
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Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
Bölümler
- 00:04Introduction
- 02:04Isolation of the S-100 Fraction of the Liver
- 03:36Trifluoroacetylation of S-100
- 05:45Determination of CD4+ T cell Immune Responses
- 07:26Isolation of Infiltrating Immune Cells from Immunized Mice
- 09:27Results: Cell Immune Responses
- 10:45Conclusion
We describe an in vivo immunization, translational hepatitis model in BALB/c mice that can be utilized to study the pathogenesis of drug-induced autoimmune hepatitis including sex differences seen in this disease. We will describe how this model demonstrates reproducible analyses using in vivo and in vitro experimental techniques.
