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直接注射慢病毒载体突出大鼠脊髓中的多种运动途径
JoVE Journal
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JoVE Journal Nörobilim
Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
Please note that all translations are automatically generated. Click here for the English version.

直接注射慢病毒载体突出大鼠脊髓中的多种运动途径

8,288 Views

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07:57 min

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March 15, 2019

DOI:

10.3791/59160-v

DOI:
10.3791/59160-v

•

07:57 min
March 15, 2019

•

5 Views
, , , ,
1Shriner’s Pediatric Research Center,Temple University

Özet

Automatically generated

该协议演示了在大鼠脊髓组织中注射一种具有可追溯性的可转移性病毒载体。载体在突触处被占据, 并被输送到目标神经元的细胞体。该模型适用于重要脊柱通路的逆行追踪或基因治疗应用中的靶向细胞。

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Cite this Article

Keefe, K. M., Junker, I. P., Sheikh, I. S., Campion, T. J., Smith, G. M. Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord. J. Vis. Exp. (145), e59160, doi:10.3791/59160 (2019).

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