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Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
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JoVE Journal Neuroscience
Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord

Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord

DOI:
10.3791/59160-v

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07:57 min

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March 15, 2019

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, , , ,
1Shriner’s Pediatric Research Center,Temple University

Chapters

  • 00:04Title
  • 00:45Surgical Preparation
  • 02:13Vertebral Column Exposure
  • 03:44Laminectomy
  • 04:36Virus Loading
  • 05:48Virus Injection and Post-Operative Care
  • 06:42Results: Representative Transduction of Neurons in the Spinal Cord and Brainstem
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates injection of a retrogradely transportable viral vector into rat spinal cord tissue. The vector is taken up at the synapse and transported to the cell body of target neurons. This model is suitable for retrograde tracing of important spinal pathways or targeting cells for gene therapy applications.

Tags

Keywords: Lentiviral VectorSpinal CordMotor PathwaysViral VectorGenetic MaterialNeural CircuitsDirect InjectionL1 To L4 Spinal LevelsMicromanipulatorAnesthetized RatSkin IncisionSpinous ProcessesRat Tooth Forceps

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