Dergi
/
Biyokimya
/
体外和体内实验的位定向诱变-以大肠杆菌rna相互作用为例
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Please note that all translations are automatically generated. Click here for the English version.

体外和体内实验的位定向诱变-以大肠杆菌rna相互作用为例

19,255 Views

•

07:04 min

•

February 05, 2019

DOI:

10.3791/58996-v

DOI:
10.3791/58996-v

•

07:04 min
February 05, 2019

•

22 Views
, ,
1Biyokimya ve Moleküler Biyoloji Anabilim Dalı,University of Southern Denmark

Özet

Automatically generated

位定向诱变是一种用于引入脱氧核糖核酸 (dna) 中的特定突变的技术。该协议描述了如何进行现场定向诱变与2步和3步聚合酶链反应 (pcr) 为基础的方法, 这是适用于任何 dna 片段的利益。

Explore More Videos

Site-directed MutagenesisRNA InteractionsEscherichia ColiPCRDNA TemplatePrimer PairsAgarose Gel ElectrophoresisDNA PurificationSpectrophotometry2-step PCR3-step PCRRNA-RNA InteractionsRNA-protein Interactions

Read Article

Cite this Article

Andreassen, P. R., Pettersen, J. S., Jørgensen, M. Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli. J. Vis. Exp. (144), e58996, doi:10.3791/58996 (2019).

Download .ris file

Copy

Share Video

.

Copy