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Culture de cellules primaires de neurones GABAergiques ou glutamatergiques purifiés établis par tri cellulaire activé par fluorescence
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
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Culture de cellules primaires de neurones GABAergiques ou glutamatergiques purifiés établis par tri cellulaire activé par fluorescence

10,820 Views

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10:57 min

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June 06, 2019

DOI:

10.3791/58974-v

DOI:
10.3791/58974-v

•

10:57 min
June 06, 2019

•

5 Views
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1Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

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Ce protocole décrit une méthode basée sur le tri cellulaire pour la purification et la culture des neurones GABAergiques fluorescents ou glutamatergiques du néocortex et de l’hippocampe de souris ou de rats postnatals.

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Turko, P., Groberman, K., Kaiser, T., Yanagawa, Y., Vida, I. Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting. J. Vis. Exp. (148), e58974, doi:10.3791/58974 (2019).

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