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荧光活化细胞分选建立纯化的 Gabaep 或谷胱甘肽神经元的原代细胞培养
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JoVE Journal Nörobilim
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
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荧光活化细胞分选建立纯化的 Gabaep 或谷胱甘肽神经元的原代细胞培养

10,820 Views

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10:57 min

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June 06, 2019

DOI:

10.3791/58974-v

DOI:
10.3791/58974-v

•

10:57 min
June 06, 2019

•

5 Views
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1Institut für Integrative Neuroanatomie, Charité,Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center,Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience,Graduate School of Medicine, Gunma University

Özet

Automatically generated

该协议描述了一种基于细胞分选的方法, 用于从产后小鼠或大鼠的新皮层和海马中纯化和培养荧光 gabaep 或谷氨酸神经元。

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Cite this Article

Turko, P., Groberman, K., Kaiser, T., Yanagawa, Y., Vida, I. Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting. J. Vis. Exp. (148), e58974, doi:10.3791/58974 (2019).

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