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A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum
JoVE Journal
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JoVE Journal Nörobilim
A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum

A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum

DOI:
10.3791/58953-v

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09:04 min

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January 17, 2019

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, ,
1Department of Pathology and Cell Biology,Columbia University, 2Division of Movement Disorders, Department of Neurology,Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health,Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine,Yale University

Bölümler

  • 00:04Başlık
  • 00:33Prepare Cryostat for Tissue Sectioning
  • 01:51Sectioning Tissue
  • 03:50Fixing Tissue/Stain-less Visualization
  • 04:47Laser Capture Microdissection of Purkinje Cells
  • 06:09Results: RNA Isolation from Laser Capture Microdissected Purkinje Cells
  • 07:59Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol uses a stain-free approach to visualize and isolate Purkinje cells in fresh-frozen tissue from human post-mortem cerebellum via laser capture microdissection. The purpose of this protocol is to generate sufficient amounts of high-quality RNA for RNA-sequencing.

Etiketler

RNA IsolationLaser Capture MicrodissectionPurkinje CellsPost-mortem Human CerebellumStain-free VisualizationCryostatOCTRNase DecontaminationTemperature Control

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