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Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae
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Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae
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January 07, 2019
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Zebrafish (Danio rerio) are becoming a widely-used vertebrate animal model for microbial colonization and pathogenesis. This protocol describes the use of the protozoan Paramecium caudatum as a vehicle for food-borne infection in zebrafish larvae. P. caudatum readily internalizes bacteria and get taken up by larval zebrafish through natural preying behavior.
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Flores, E., Thompson, L., Sirisaengtaksin, N., Nguyen, A. T., Ballard, A., Krachler, A. Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae. J. Vis. Exp. (143), e58949, doi:10.3791/58949 (2019).
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