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電気化学と蛍光顕微鏡を用いたプロトン ポンプ膜酵素の研究のための単一リポソーム測定
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JoVE Journal Biyokimya
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
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電気化学と蛍光顕微鏡を用いたプロトン ポンプ膜酵素の研究のための単一リポソーム測定

7,287 Views

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12:15 min

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February 21, 2019

DOI:

10.3791/58896-v

DOI:
10.3791/58896-v

•

12:15 min
February 21, 2019

•

11 Views
, ,
1School of Biomedical Sciences & the Astbury Centre for Structural Molecular Biology,University of Leeds, 2Department of Chemistry and Nano-Science Center,University of Copenhagen

Özet

Automatically generated

ここでは、プロトコルを提案単一リポソームの脂質膜を渡るプロトン透過の分子機構の研究例としてシトクロムbo3を使用します。電気化学、蛍光顕微鏡、単一小胞、含む単一または複数の酵素のルーメン内の pH の変化を組み合わせることを検出および分析できる個別に。

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Mazurenko, I., Hatzakis, N. S., Jeuken, L. J. Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy. J. Vis. Exp. (144), e58896, doi:10.3791/58896 (2019).

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