Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Ievgen Mazurenko; Nikos S. Hatzakis; Lars J.C. Jeuken

doi:10.3791/58896

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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

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生化学

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JoVE Journal 生化学

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

7,288 Views

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12:15 min

•

February 21, 2019

DOI:

10.3791/58896-v

DOI:

10.3791/58896-v

•

12:15 min

February 21, 2019

•

11 Views

Ievgen Mazurenko¹, Nikos S. Hatzakis², Lars J.C. Jeuken¹

¹School of Biomedical Sciences & the Astbury Centre for Structural Molecular Biology,University of Leeds, ²Department of Chemistry and Nano-Science Center,University of Copenhagen

概要

Here, we present a protocol to study the molecular mechanism of proton translocation across lipid membranes of single liposomes, using cytochrome bo₃ as an example. Combining electrochemistry and fluorescence microscopy, pH changes in the lumen of single vesicles, containing single or multiple enzyme, can be detected and analyzed individually.

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Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

概要

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