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In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
Bölümler
- 00:04Başlık
- 00:33Immobilizing Larva to Imaging Chamber
- 02:06Injection of α-Bungarotoxin into the Heart Cavity to Paralyze Larva
- 03:47Alignment of Larva and Fluid-jet
- 07:03Single-plane Imaging Acquisition
- 07:34Results: Spatiotemporal Visualization of Presyntaptic GCaMP6s Signals During Fluid-jet Stimulation
- 08:22Conclusion
The zebrafish is a model system that has many valuable features including optical clarity, rapid external development, and, of particular importance to the field of hearing and balance, externally located sensory hair cells. This article outlines how transgenic zebrafish can be used to assay both hair-cell mechanosensation and presynaptic function in toto.
