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An Unbiased Approach of Sampling TEM Sections in Neuroscience
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Nörobilim
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JoVE Journal Nörobilim
An Unbiased Approach of Sampling TEM Sections in Neuroscience

An Unbiased Approach of Sampling TEM Sections in Neuroscience

DOI:
10.3791/58745-v

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10:56 min

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April 13, 2019

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, , , , , , , , , , , ,
1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center,Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center,Medical University of Graz, 3Division of General Neurology, Department of Neurology,Medical University of Graz, 4Institute of Pathology,Medical University of Graz, 5Department of Pathology, Medical Faculty,Otto von Guericke University Magdeburg

Bölümler

  • 00:04Başlık
  • 01:02TEM Preparation Embedding, Ultra-Thin Sectioning and Staining
  • 02:05Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages
  • 05:25Analyze the TEM images with ImageJ to Document Ultrastructural Features
  • 07:10Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph
  • 09:19Results: Unbiased Approach of Sampling TEM Sections
  • 10:25Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We introduce a novel workflow for electron microscopy investigations of brain tissue. The method allows the user to examine neuronal features in an unbiased fashion. For elemental analysis, we also present a script that automatizes most of the workflow for randomized sampling.

Etiketler

– Unbiased Sampling- Electron Microscopy- Neuroscience- Quantification- Numerical Density- Synapses- Neural Network- Sample Preparation- Ultramicrotome- Serial Sections- Slot Grid- Counterstaining- Transmission Electron Microscope (TEM)- TEM Serial Section Software- Region Of Interest (ROI)- Random Point Sampling

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