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Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

DOI:
10.3791/58739-v

•

08:53 min

•

January 12, 2019

•

, , ,
1Sainsbury Laboratory,University of Cambridge

Bölümler

  • 00:04Başlık
  • 00:47Plant Growth and Sample Preparation
  • 02:09Gibberellin4 (GA4) Treatment
  • 03:45Tissue of Interest Time Course Setup
  • 05:093-D Image Analysis
  • 06:36Results: Representative Endogenous and Exogenous GA Gradients in Arabidopsis Roots and Dark-grown Hypocotyls
  • 07:58Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Gibberellin Perception Sensor 1 (GPS1) is the first Förster resonance energy transfer-based biosensor for measuring the cellular levels of gibberellin phytohormones with a high spatiotemporal resolution. This protocol reports on the method to visualize and quantify cellular gibberellin levels using the genetically encoded nlsGPS1 biosensor in Arabidopsis hypocotyls and root tips.

Etiketler

NlsGPS1FRETArabidopsisPhytohormoneGibberellinCellular ImagingBiosensorPlant DevelopmentRatiometric ImagingMurashige And Skoog AgarStratificationGrowth ChamberHypocotylSteady State MeasurementsGA4Vacuum GreaseMicroscope SlideCover Slip

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