/
/
Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
This content is Free Access.
Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
10,323 Views
•
08:53 min
•
January 12, 2019
DOI:
Özet
Automatically generated
Gibberellin Perception Sensor 1 (GPS1) is the first Förster resonance energy transfer-based biosensor for measuring the cellular levels of gibberellin phytohormones with a high spatiotemporal resolution. This protocol reports on the method to visualize and quantify cellular gibberellin levels using the genetically encoded nlsGPS1 biosensor in Arabidopsis hypocotyls and root tips.
Read Article
Cite this Article
Rizza, A., Walia, A., Tang, B., Jones, A. M. Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor. J. Vis. Exp. (143), e58739, doi:10.3791/58739 (2019).
Copy