Dergi
/
Biyoloji
/
A BW Reporter System for Studying Receptor-Ligand Interactions
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
A BW Reporter System for Studying Receptor-Ligand Interactions

A BW Reporter System for Studying Receptor-Ligand Interactions

DOI:
10.3791/58685-v

•

06:05 min

•

January 07, 2019

•

, , ,
1Department of Hematology,Hadassah – Hebrew University Medical Center, 2The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC),Hadassah – Hebrew University Medical Center

Bölümler

  • 00:04Başlık
  • 00:36Transfection of the Plasmid that Expresses the Chimeric Protein into BW Cells
  • 03:10Incubation of the Transfected BW Cells with Targets
  • 03:32ELISA
  • 04:38Results: BW Reporter System Demonstrated
  • 05:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes how to establish a reporter system that can be used to identify and quantify receptor-ligand interactions.

Etiketler

BW Reporter SystemReceptor-ligand InteractionsLigandEndogenous LigandTransfectionPcDNA3 PlasmidSodium AcetateEthanol PrecipitationElectroporationG418 Selection96-well PlateELISA

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Read Article