Here, we present a protocol to perform ethanol extraction of lignin from several biomass sources. The effect of the extraction conditions on the lignin yield and β-O-4 content are presented. Selective depolymerization is performed on the obtained lignins to obtain high aromatic monomer products.
Zijlstra, D. S., de Santi, A., Oldenburger, B., de Vries, J., Barta, K., Deuss, P. J. Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield. J. Vis. Exp. (143), e58575, doi:10.3791/58575 (2019).