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Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
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JoVE Journal Nörobilim
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

34,471 Views

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08:17 min

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February 15, 2019

DOI:

10.3791/58565-v

DOI:
10.3791/58565-v

•

08:17 min
February 15, 2019

•

47 Views
, , , ,
1Department of Neurology,Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology

Özet

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Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.

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Li, D., Li, Y., Tian, Y., Xu, Z., Guo, Y. Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice. J. Vis. Exp. (144), e58565, doi:10.3791/58565 (2019).

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