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A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
JoVE Journal
Çevre
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JoVE Journal Çevre
A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
DOI:

05:03 min

October 29, 2018

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'Bölümler'

  • 00:04'Başlık'
  • 00:41Preparing Ready-to-Use B. tabaci LAMP Kit
  • 01:39DNA Extraction and B. tabaci LAMP Assay
  • 02:58LAMP Assay Read-out
  • 03:25Results: Intercepted B. tabaci Insect Specimens Analyzed and Validated via LAMP Assay
  • 04:20Conclusion

Özet

'Otomatik Çeviri'

This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.

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