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Purification of Low-abundant Cells in the Drosophila Visual System
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Genetik
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JoVE Journal Genetik
Purification of Low-abundant Cells in the Drosophila Visual System

Purification of Low-abundant Cells in the Drosophila Visual System

DOI:
10.3791/58474-v

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07:09 min

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September 26, 2018

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, ,
1Department of Neurobiology,Harvard Tıp Fakültesi

Bölümler

  • 00:04Başlık
  • 00:30Papain and Proteolytic Enzyme Blend
  • 01:49Dissections
  • 02:44Cell Dissociation
  • 05:45Results: Effective Purification of Low-abundant Drosophila Cells
  • 06:35Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a cell dissociation protocol for efficiently isolating cells present at low abundance within the Drosophila visual system through fluorescence activated cell sorting (FACS).

Etiketler

Cell PurificationDrosophila Visual SystemTranscriptomic AnalysisGenomic AnalysisPapainProteolytic EnzymeOptic LobesMicrotubeDissectionLow-abundant Cells

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