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Een cel-gebaseerde Assay te onderzoeken niet-spier Myosin II Contractility via de gevouwen-gastrulatie signalering traject in Drosophila S2R + cellen
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JoVE Journal Gelişim Biyolojisi
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
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Een cel-gebaseerde Assay te onderzoeken niet-spier Myosin II Contractility via de gevouwen-gastrulatie signalering traject in Drosophila S2R + cellen

7,227 Views

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07:15 min

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August 19, 2018

DOI:

10.3791/58325-v

DOI:
10.3791/58325-v

•

07:15 min
August 19, 2018

•

1 Views
, , , , , , ,
1Biyoloji Bölümü,The University of North Carolina at Chapel Hill, 2Biyoloji Bölümü,Reed College, 3Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

Özet

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Hier beschrijven we een test van de contractility met behulp van Drosophila S2R + cellen. De toepassing van een exogene ligand, gevouwen gastrulatie (Fog), leidt tot het activeren van de mist signalering traject en mobiele contractility. Deze test kan worden gebruikt om te onderzoeken van de regulering van cellulaire contractility eiwitten in de mist signalering traject.

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Peters, K. A., Detmar, E., Sepulveda, L., Del Valle, C., Valsquier, R., Ritz, A., Rogers, S. L., Applewhite, D. A. A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells. J. Vis. Exp. (138), e58325, doi:10.3791/58325 (2018).

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