Dergi
/
Gelişim Biyolojisi
/
果蝇S2R+ 细胞折叠-肠信号通路检测非肌球蛋白 II . 收缩力的细胞基础研究
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
Please note that all translations are automatically generated. Click here for the English version.

果蝇S2R+ 细胞折叠-肠信号通路检测非肌球蛋白 II . 收缩力的细胞基础研究

7,223 Views

•

07:15 min

•

August 19, 2018

DOI:

10.3791/58325-v

DOI:
10.3791/58325-v

•

07:15 min
August 19, 2018

•

1 Views
, , , , , , ,
1Biyoloji Bölümü,The University of North Carolina at Chapel Hill, 2Biyoloji Bölümü,Reed College, 3Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

Özet

Automatically generated

这里我们描述了一种使用果蝇S2R+ 细胞的收缩力测定方法。外源配体, 折叠肠 (雾) 的应用, 导致了雾信号通路和细胞收缩力的活化。该方法可用于研究雾信号通路中细胞收缩蛋白的调节。

Explore More Videos

Non-muscle Myosin IIContractilityFolded-gastrulation Signaling PathwayDrosophila S2R+ CellsCell-based AssayCell DensityCell MorphologyCell Shape ChangeMorphogenesisDevelopmentGene DiscoveryRNAi Depletion

Read Article

Cite this Article

Peters, K. A., Detmar, E., Sepulveda, L., Del Valle, C., Valsquier, R., Ritz, A., Rogers, S. L., Applewhite, D. A. A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells. J. Vis. Exp. (138), e58325, doi:10.3791/58325 (2018).

Download .ris file

Copy

Share Video

.

Copy