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Generation von Knock-out-Grund- und erweiterten menschlichen NK-Zellen mit Cas9 Ribonucleoproteins
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JoVE Journal İmmünoloji ve Enfeksiyon
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
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Generation von Knock-out-Grund- und erweiterten menschlichen NK-Zellen mit Cas9 Ribonucleoproteins

12,177 Views

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07:20 min

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June 14, 2018

DOI:

10.3791/58237-v

DOI:
10.3791/58237-v

•

07:20 min
June 14, 2018

•

5 Views
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1Center for Childhood Cancer and Blood Disease,Nationwide Children’s Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, 3Center for Clinical and Translational Research,Nationwide Children’s Hospital Research Institute

Özet

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Hier präsentieren wir Ihnen ein Protokoll zur primären oder erweiterten menschlichen natürlichen (NK) Killerzellen mit Cas9 Ribonucleoproteins (Cas9/RNPs) genetisch zu verändern. Mithilfe dieses Protokolls erzielten wir menschliche NK-Zellen für Umwandlung Wachstumsfaktor-b-Rezeptor 2 (TGFBR2) und Hypoxanthin-Phosphoribosyltransferase 1 (HPRT1) mangelhaft.

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Naeimi Kararoudi, M., Dolatshad, H., Trikha, P., Hussain, S. A., Elmas, E., Foltz, J. A., Moseman, J. E., Thakkar, A., Nakkula, R. J., Lamb, M., Chakravarti, N., McLaughlin, K. J., Lee, D. A. Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins. J. Vis. Exp. (136), e58237, doi:10.3791/58237 (2018).

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