Dergi
/
Biyomühendislik
/
Microbioreactor uso de alto rendimiento automático para la producción de IgG1 modelo en células CHO
JoVE Journal
Biyomühendislik
This content is Free Access.
JoVE Journal Biyomühendislik
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
Please note that all translations are automatically generated. Click here for the English version.

Microbioreactor uso de alto rendimiento automático para la producción de IgG1 modelo en células CHO

10,595 Views

•

08:15 min

•

September 28, 2018

DOI:

10.3791/58231-v

DOI:
10.3791/58231-v

•

08:15 min
September 28, 2018

•

8 Views
, , , , , , , ,
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Özet

Automatically generated

Se presenta un protocolo detallado para la operación concurrente de culturas de célula paralelo 48 bajo variadas condiciones en un sistema de microbioreactor. Proceso de cultivo celular, la cosecha y el análisis del título de anticuerpos posterior se describen.

Explore More Videos

High-throughputAutomated Microbioreactor SystemIgG1 ProductionCHO CellsCell Line CharacterizationProcess OptimizationShake FlasksAutomationSmall-scale StudiesCell CultureSubculturingCell CounterMicrobioreactor SoftwareTemperature ControlDissolved OxygenPH Monitoring

Read Article

Cite this Article

Velugula-Yellela, S. R., Kohnhorst, C., Powers, D. N., Trunfio, N., Faustino, A., Angart, P., Berilla, E., Faison, T., Agarabi, C. Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells. J. Vis. Exp. (139), e58231, doi:10.3791/58231 (2018).

Download .ris file

Copy

Share Video

.

Copy